User:Jarle Pahr/Electrophoresis

Notes on gel electrophoresis

Plasmid DNA that has not been cut with a restriction endonuclease will migrate in 3 forms: supercoiled closed circular DNA, single-strand nicked open circle DNA, and sheared linear DNA. http://www.sfu.ca/biology/courses/bisc431/lab3.htm

Supercoiled plasmid DNA will migrate faster than linear forms.

The effect of loading dye may cause mismatch between sample DNA bands and the DNA ladder bands.

http://www.protocol-online.org/biology-forums/agarose.html

Supplies

Lysplate: http://www.kptkomet.no/produkter/no_89/biologi/vnr/790510/

Equipment

WVR:

Thermo scientific:

http://www.thermoscientific.com/content/tfs/en/product/owl-easycast-b1-mini-gel-electrophoresis-systems.html

Carolina:

Visualization

http://www.bluetransilluminator.com/