Difference between revisions of "User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/04/10"

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==Entry title==
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===Primer design===
This document describes the standardization of pK18mobsacB, pRK404 & pBBRMCS for iGEM proposes.
 
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The sequence of pSB1T3 was obtained form the Registry. This plasmid backbone can be found in 2010 Kit Plate 1 Well 7A. This plasmid instance carries a Red fluorescent protein generator denominated BBa_J04450, I also obtained its sequence from the Registry.
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I have merged the sequences to design primers that would allows us to amplify a plasmid region. This plasmid region amplified will be used to standardize the vectors pK18mobsacB, pRK404 & pBBRMCS.
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Method.
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1)
  
  

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Primer design

The sequence of pSB1T3 was obtained form the Registry. This plasmid backbone can be found in 2010 Kit Plate 1 Well 7A. This plasmid instance carries a Red fluorescent protein generator denominated BBa_J04450, I also obtained its sequence from the Registry.

I have merged the sequences to design primers that would allows us to amplify a plasmid region. This plasmid region amplified will be used to standardize the vectors pK18mobsacB, pRK404 & pBBRMCS.

Method.

1)