Difference between revisions of "User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/03/10"

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==Entry title==
 
==Entry title==
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Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells.
 
Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells.
  

Revision as of 15:34, 10 March 2011

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Distribution 2009 Plate 1 Well A2 transformed into DB3.1 CaCl2 competent cells.

2µl of DNA

100µl Cells

1)30min on ice

2)2 min 42ºC

3)5 min on ice

4)Add 900 µl LB

5)1 hr. 37ºC

6)Plate on LB Cb or Amp

7)Incubate 37ºC overnight >16 gr.