User:J. M. Uriel Urquiza/Notebook/pRK404 & pK18Mobsacb standardization/2011/04/10: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 6: | Line 6: | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ===Primer design=== | ||
The sequence of pSB1T3 was obtained form the Registry. This plasmid backbone can be found in 2010 Kit Plate 1 Well 7A. This plasmid instance carries a Red fluorescent protein generator denominated BBa_J04450, I also obtained its sequence from the Registry. | |||
I have merged the sequences to design primers that would allows us to amplify a plasmid region. This plasmid region amplified will be used to standardize the vectors pK18mobsacB, pRK404 & pBBRMCS. | |||
Method. | |||
1) | |||
Revision as of 21:15, 10 April 2011
 Project name
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> |
Primer designThe sequence of pSB1T3 was obtained form the Registry. This plasmid backbone can be found in 2010 Kit Plate 1 Well 7A. This plasmid instance carries a Red fluorescent protein generator denominated BBa_J04450, I also obtained its sequence from the Registry. I have merged the sequences to design primers that would allows us to amplify a plasmid region. This plasmid region amplified will be used to standardize the vectors pK18mobsacB, pRK404 & pBBRMCS. Method. 1)
| |
