Difference between revisions of "User:Heather N. Currey/Notebook/Taylor C. elegans Lab Notebook/2013/05/17"

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(17 May 2013)
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==17 May 2013==
 
==17 May 2013==
*
 
  
Lab Meeting - see [https://docs.google.com/a/alaska.edu/document/d/1WEjKBAGd4NUJLZjGYykSZDrkruUn0_0Nxk0Gc3k3cMU/edit?usp=sharing Memos - Senile Worms]
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*Lab Meeting - see [https://docs.google.com/a/alaska.edu/document/d/1WEjKBAGd4NUJLZjGYykSZDrkruUn0_0Nxk0Gc3k3cMU/edit?usp=sharing Memos - Senile Worms]
Chunk ID1:B21 worms to new plate
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*Chunk ID1:B21 worms to new plate
 
       -plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon!
 
       -plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon!
ROS assay with Courtney and Skyler
+
*ROS assay with Courtney and Skyler
 
       -transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms  
 
       -transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms  
 
       -washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces  
 
       -washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces  
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       -used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye
 
       -used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye
 
       RESULTS
 
       RESULTS
Inoculated OP50 for seeding plates
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*Inoculated OP50 for seeding plates
 
       -10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013
 
       -10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013
 
       -8 ml LB w/ 4µl strep. for stock plates  
 
       -8 ml LB w/ 4µl strep. for stock plates  
  
Revaluated Schedule for RNAi treatments [https://docs.google.com/a/alaska.edu/spreadsheet/ccc?key=0At5kTTSjupzTdHhuMldJbEQtMWpBSlpTSXh0aGw4TGc&usp=sharing RNAi Screen Schedule]
+
*Revaluated Schedule for RNAi treatments [https://docs.google.com/a/alaska.edu/spreadsheet/ccc?key=0At5kTTSjupzTdHhuMldJbEQtMWpBSlpTSXh0aGw4TGc&usp=sharing RNAi Screen Schedule]
  
  

Revision as of 14:12, 17 May 2013

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17 May 2013

     -plate used was stock NGM plate that was starting to dry around the edges with a healthy lawn, I will need to seed new stock plates soon!
  • ROS assay with Courtney and Skyler
     -transfered 50 worms per microcentrifuge tube w/100 µl M9 solution, I did 2 tubes using ZDIS5 worms, Skyler did 3 tubes w/ ID1;B21 worms 
     -washed tubes 3x with 50µl M9 to remove bacteria - autofluoresces 
     -moved samples onto 96 well plate in sections F1-3 to H1-3
     -used plate reader in Dunlap Lab to measure fluorescence after treatment w/dye
     RESULTS
  • Inoculated OP50 for seeding plates
     -10ml LB -> 1.5 ml w/ 5µl of strep. for experimental plates to be used on 21/May/2013
     -8 ml LB w/ 4µl strep. for stock plates