Difference between revisions of "User:Guido/Notes/July 07"

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Line 6: Line 6:
  
 
** 3 μl culture
 
** 3 μl culture
** 5 μl taq buffer
 
 
** 1 μl f-primer
 
** 1 μl f-primer
 
** 1 μl r-primer
 
** 1 μl r-primer
** .5 μl taq
+
** 45 μl supermix
** 39.5 dH20
 
 
** 50  μl reaction
 
** 50  μl reaction
  
  
  
  * 94 4 min
+
  * 95 10 min
 
   
 
   
  * 94 30 sec   
+
  * 95 30 sec   
  * 53 30 sec
+
  * 51 30 sec
 
  * 72  2 min
 
  * 72  2 min
 +
* X30
 
   
 
   
* 94  30 sec
+
  * 72  7 min
* 53  30 sec
 
* 72  2 min
 
 
  * 72  5 min
 
 
  * 4 forever
 
  * 4 forever

Latest revision as of 08:35, 3 July 2007

July 3, 2007

  • Do a culture pcr from last recombination of specR to replace MutS


    • 3 μl culture
    • 1 μl f-primer
    • 1 μl r-primer
    • 45 μl supermix
    • 50 μl reaction


* 95  10 min

* 95  30 sec  
* 51  30 sec
* 72  2 min
* X30

* 72   7 min
* 4 forever