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Today's objective is to perform a UV-Vis and a fluorescence spectroscopy on the three solutions we have been preparing last 2 weeks. The main purpose of these measures is to quantify the amount of dye and Gold NPs in each solution and to assess for the efficiency of the binding reaction.
The UV-Vis spectroscopy is going to allow us to quantify dye in each solution, using the fact that our dye absorb at 602nm. To do so, we are going to make a spectrum of the solution by using a spectrophotometer (UV-1800 Shimadzu) in the wavelength range of 200-800nm. We'll then determine the dye/BSA molecule ratio using the initial amount of BSA (we assume that no BSA has been lost during the experiment). The fluorescence spectroscopy will allow us to compare how efficient the dying process is; to do so we'll compare fluorescence spectrum btw our three different solutions. We are going to use the fact that our dye absorbs at 602nm and emits at 672nm.