Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:DNA methylation"

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(Depurination of DNA)
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=== PH dependent enzymatic kinetics ===
* competitive inhibitor vs no inhibitor (-> Adenosine deaminase)
* dNTP: nucleotide (purine) metabolism, may be involved in N (phosphohistidine) metabolism. [http://www.themedicalbiochemistrypage.org/nucleotide-metabolism.php Verify this information here.]
* MSG (Monosodium glutamate): a potent source of l-glutamine acid, thus an '''ortho''' (a non-competitive substrate?) ligand for PH-dependent enzymatic reactions.
=== Depurination of DNA ===
* [[User:Etienne Robillard|Etienne Robillard]] 08:05, 15 February 2013 (EST) About E.coli, How can a certified biohacker crack human dna with purine analogs and insert novel genes (with homologous recombination techniques) in the e.coli genome without feeling any regrets or shame for the e.coli offsprings? ([https://openwetware.org/wiki/Ethanol_precipitation_of_nucleic_acids_protocol_-_source_code Source code example is here])
** [[User:Etienne Robillard|Etienne Robillard]] 15:44, 15 February 2013 (EST) DNA script kiddies, generally speaking, don't feel much guiltiness in breaking DNA per se: In they're vision of the world the E. coli genome is programmable .
*** [[User:Etienne Robillard|Etienne Robillard]] 16:14, 15 February 2013 (EST) Agent E. coli: Heck, is the term '''DNA cracking''' appropriate to describe high-profile DNA vandalism performed by rogue biohackers ?
=== Keywords ===
E. coli K-12, MG1655, genomics, ADA, purine, oligonucleotides, Fmoc 
=== See also ===
Cat eye syndrome (CES)
=== References ===
* N. C. Price, L. Stevens, Fundamentals of Enzymology, Second Edition. Oxford Science Publications, 1989. ISBN 0198552963

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