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== Project Bare Back Mountain: Decrypting the Eugenicist Connection hiden between the crypto-language of Synthetic Biology and Agent E.coli ==
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'''"For them, there is no God; the Government is the supreme principle deciding above all consideration of intangible moral. The family, seen as a "bourgeois institution" must be destroyed and replaced by a governmental control of reproduction and education, the Lebensborn. This is how the Government can control the "production" of human beings in the future, both in the quantitative and "qualitative" plan(8)."''' [http://www.trdd.org/EUGBR_2E.HTM#_Toc511642187 1]
 
 
 
__TOC__
 
 
 
=== Synopsis ===
 
 
 
What are the public health risks imposed by the chemtrails agenda and why ? Is the spraying of biologically active aerosols over humans a delusional
 
conspiracy theory or a landmark of organized crime to expose the human DNA to potentially harmful genetic material? Are chemtrails motivated by hate
 
and anger of the human genome or by a eugenics movement dedicated to transforming the human DNA into profitable commercial products ?
 
 
 
The aim of this page is to collect data on so-called '''genetic switches''', or synthetic '''transcriptional devices''' and rationalize the evidences to justify the validity
 
of my concerns on the function of the chemtrails repeated spraying atop of my house and city, in Quebec and elsewhere.
 
 
 
=== Evidence collection ===
 
 
 
==== Input/output promoters ====
 
 
 
{| border="1" cellpadding="2" class="wikitable"
 
|-
 
! scope="col" | Promoter name
 
! scope="col" | Promoter type
 
! scope="col" | Description
 
! scope="col" | Target organisms
 
! scope="col" | Restriction site 
 
! scope="col" | EC number
 
! scope="col" | References
 
! scope="col" | Biobrick Part
 
|-
 
! scope="row" | L-arabinose
 
| aldopentose
 
| Inducible pBAD/araC promoter
 
| E.coli (K-12/MG1655), P.fluorescens(?), Homo sapiens
 
| araBAD (''araC'')
 
|
 
| [http://openwetware.org/wiki/Arabinose 1], [http://en.wikipedia.org/wiki/Arabinose 2], [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6976.2010.00226.x/abstract 3], [http://www.ncbi.nlm.nih.gov/pubmed/12080425?dopt=Abstract 4], [http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/regulation/AraC.html 5]
 
| BBa_I0500
 
|-
 
! scope="row" | cAMP
 
| second messenger
 
| cyclic adenosine monophosphate is required to activate the Lac operon along with the araC promoter.
 
| Homo sapiens
 
| LacZ
 
| 3.1.4.17, 3.1.4.53, 4.6.1.1
 
| [http://en.wikipedia.org/wiki/Cyclic_adenosine_monophosphate 1]
 
|   
 
|}
 
 
 
==== Plasmid addiction systems ====
 
 
 
{| border="1" cellpadding="2" class="wikitable"
 
|-
 
! scope="col" | Plasmid name
 
! scope="col" | Origin of replication
 
! scope="col" | Organism (Strain)
 
! scope="col" | Anti-bacterial resistance
 
! scope="col" | Quorum sensing protein expression 
 
! scope="col" | ColE1 (Colicins) protein expression
 
! scope="col" | 6-'''his'''-tagged promoter
 
! scope="col" | References
 
|-
 
| scope="row" | pSB1A[C]3 (formerly pSB1A2?)
 
| scope="row" | pUC19-derived (pMB1)
 
| scope="row" | ''E.coli'' ([http://cgsc.biology.yale.edu/Strain.php?ID=111773 BW27783])
 
| scope="row" | ampicilin (ampR)
 
| scope="row" | Yes (GFP)
 
| scope="row" | Yes (ccdB?)
 
| scope="row" | Yes (L-arabinose inducible)
 
| scope="row" | [http://partsregistry.org/Part:pSB1A2 1], [http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP 2], [http://partsregistry.org/Part:BBa_I746914 3], [http://partsregistry.org/Part:BBa_I0500 4], [http://partsregistry.org/wiki/index.php?title=Part:BBa_P1010 5]
 
|-
 
| scope="row" | pSB3K3-1010
 
| scope="row" | p15a-derived (pMR101)
 
| scope="row" | ''E.coli'' (MG1655)
 
| scope="row" | kanamycin (kanR)
 
| scope="row" | Yes (GFP/RFP via F2620)
 
| scope="row" | Yes (ccdB?)
 
| scope="row" | Yes (L-arabinose inducible)
 
| [http://partsregistry.org/cgi/partsdb/related.cgi?part=F2620 1], [http://partsregistry.org/Part:BBa_F2620 2], [http://partsregistry.org/Part:BBa_V1000:Design 3]
 
|}
 
 
 
==== Reagents ====
 
 
 
[data here...]
 
 
 
==== Discussion ====
 
 
 
*  A human is not a computer program and never will be. Love is not programmable and neither are emotions which makes life beautiful or harsh. It is through the eyes of natural-born scientists that humans may learn how to live in respect with the outer world and not the opposite. Yet I find scientific progress brilliant and at the same time horrified by the efforts made to confine the human species into a predictable sequence of digits. -- [[User:Etienne Robillard|Etienne Robillard]] 21:45, 15 October 2012 (EDT)
 
* Whether the [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1681472/ T7.1 bacteriophage] was designed for E.coli or H.sapiens does not seem to limit its capacity to genetically modify (or [http://www.ncbi.nlm.nih.gov/pubmed/18410688 refactor]) DNA using a [http://www.ncbi.nlm.nih.gov/nuccore/169656098 F-plasmid like vector]. In bacterias, an equivalent process is known as [http://www.science-projects.com/F-sex.htm bacterial conjugation].
 
 
 
=== References ===
 
<biblio>
 
#Shetty-JBiolEng-2008 pmid=18410688
 
#Atsumi-Little-PNAS-2006 pmid=17135356
 
#Lutz-Bujard-Nucleic-Acid-Res-2007 pmid=9092630
 
#Morgan-Kiss-PNAS-2002 pmid=12032290
 
#KA-Datsenko-BL-Wanner-2000 pmid=10829079
 
</biblio>
 
 
 
=== Keywords ===
 
 
 
synthetic biology, biological engineering, transcriptional logic, transcription-based logic, engineered biological systems, PoPS, BioBrick standard biological part, eugenism, genocide, kill-switch, genetic switch, metabolic regulation, DNA programming, '''transcriptional inverter''', reverse engineering, deceit, collusion, scientific misconduct, scientific responsability, demagogy, ignorance, abuse of power, chemtrails, bare backing
 

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