Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent"

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m (OmpR and LuxR multi-step catalytic gene repression systems: Detection and forensic characterization of recombinant (synthetic) DNA material in vivo using dual-input, reversible enzymatic promoters)
(Agent Ecoli:Phosphohistidine Transferase Compound)
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== Agent Ecoli:Phosphohistidine Transferase Compound ==
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== Agent Ecoli:Phosphohistidine Transferase Component ==
  
 
=== OmpR and LuxR ===
 
=== OmpR and LuxR ===
  
** Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
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* Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references.
 
 
=== Experiment ===
 
 
 
In this experiment the goal is to construct a [http://www.bio-rad.com/prd/fr/FR/LSE/PDP/18721652-4f03-4c64-90f8-ab309e058dbb/Forensic-DNA-Fingerprinting-Kit forensic DNA fingerprinting kit] to verify the DNA fragments for evidences of electrocompetent-inducible promoters and cross-species gene vectors to produce bioluminiscence agaisnt a control group (MG1655).
 
 
 
==== Experiment checklist ====
 
 
 
* Control group will need to be plasmid-free - Obtain E. coli strain mg1655 as reference model for the electrophoresis experiment.
 
* DNA extraction: data interpretation is subject to errors in the protocol - Find a coherent protocol specific for E.coli transformations using multi-plasmid vectors to transform E.coli into the desired mutant strain. 
 
* familiarize yourself with the various electrophoresis assay methods - An important resource to learn and study the basics of E.coli transcription ab initio using recombinant DNA.   
 
* more checklist fine points here... 
 
  
 
== See also ==
 
== See also ==

Revision as of 01:52, 7 November 2012

Agent Ecoli:Phosphohistidine Transferase Component

OmpR and LuxR

  • Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.

See also