Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:ToggleSwitchComponent"

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== atc vs IPTG ==
=== Overview ===
* tetR (tetracycline resistance gene) + LacR (Lactose resistance gene; LacZ) bistable toggle switch: "TL phage lambda"
* Definition of "lysogen" (Wikipedia): "A lysogen or lysogenic phage is a phage that can exist as a DNA in its dormant state (prophage) within its host organism. A prophage is either integrated into the host bacteria's chromosome or more rarely exists as a stable plasmid within the host cell. The prophage expresses gene(s) that repress the phage's lytic action, until this suppression is disrupted (see lytic cycle). Currently a variety of studies are being conducted to see whether other genes are active during lysogeny, examples of which include phage-encoded tRNA and virulence genes." [http://en.wikipedia.org/wiki/Lysogen 1]
* IPTG acts as LacR promoter => prophage positive regulation !
* aTc (tetR): a strong promoter (in contrast to araBAD/pLacZ) !!
* DNA promoter ratio/balance is important key factor to consider !!!
* 5832 combinations possible.. (TL3'?)
* grow lytically in the absence of IPTG...
* http://openwetware.org/wiki/IGEM:IMPERIAL/2007/Projects/Hrp_System/Parts
* "The original plan for our project was to model the dynamics of the spread of an epidemic within a population. We hoped to make a multi-scale model of epidemic spread and test it in a model population..."
* http://2007.igem.org/Virginia_Tech/Project
* http://rrresearch.fieldofscience.com/2007/05/time-course-and-iptg-dependence.html
* "The results may have implications for competence induction in H. influenzae too."
* B. subtilis: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC285643/
* H. influenza: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC246225/
* TL3' = GAT TCT
* strong promoter (tetR) repress weak promoter.. (lacZ)
* PC = PHP ?
* piperidine derived protection groups (Tris-HCl, Fmoc, dA, TX100) ??
* piperidine + curcuma longa = chilly and spicy ingredient with Indian flavor ! (For best results stir well piperidine with curi in a meal or soup!)

Latest revision as of 03:30, 23 August 2015

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