User:Etienne Robillard/Notebook/Agent Ecoli:ToggleSwitchComponent
< User:Etienne Robillard | Notebook
Revision as of 17:21, 26 November 2012 by Etienne Robillard (User:Etienne Robillard/Notebook/Agent Ecoli:atc vs iptg moved to User:Etienne Robillard/Notebook/Agent Ecoli:ToggleSwitchComponent)
atc vs IPTG
- tetR (tetracycline resistance gene) + LacR (Lactose resistance gene; LacZ) bistable toggle switch: "TL phage lambda"
- Definition of "lysogen" (Wikipedia): "A lysogen or lysogenic phage is a phage that can exist as a DNA in its dormant state (prophage) within its host organism. A prophage is either integrated into the host bacteria's chromosome or more rarely exists as a stable plasmid within the host cell. The prophage expresses gene(s) that repress the phage's lytic action, until this suppression is disrupted (see lytic cycle). Currently a variety of studies are being conducted to see whether other genes are active during lysogeny, examples of which include phage-encoded tRNA and virulence genes." 1
- IPTG acts as LacR promoter => prophage positive regulation !
- aTc (tetR): a strong promoter (in contrast to araBAD/pLacZ) !!
- DNA promoter ratio/balance is important key factor to consider !!!
- 5832 combinations possible.. (TL3'?)
- grow lytically in the absence of IPTG...
- "The original plan for our project was to model the dynamics of the spread of an epidemic within a population. We hoped to make a multi-scale model of epidemic spread and test it in a model population..."
- "The results may have implications for competence induction in H. influenzae too."
- B. subtilis: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC285643/
- H. influenza: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC246225/
- TL3' = GAT TCT
- strong promoter (tetR) repress weak promoter.. (lacZ)
- PC = PHP ?
- piperidine derived protection groups (Tris-HCl, Fmoc, dA, TX100) ??
- piperidine + curcuma longa = chilly and spicy ingredient with Indian flavor ! (For best results stir well piperidine with curi in a meal or soup!)