Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation"

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== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
== Disclaimer ==
== Hypothesis ==
* GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]]
=== D1210 perhaps just doesn't fit... ===
* I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
** lambda lysogen (ie HB101)
** F+ plasmid
** lacY
** hsdR restriction system (ie: leaky restriction system)
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
=== BW26434 ===
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
** Are they doing this as a habit or training to become excessively insane? 
** A Cloning ninja signature perhaps ?
** Random muslings ?
** ?
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
* PHP = amine orthogonal peptide protection group ? (see [[http://openwetware.org/wiki/CH391L/S12/Unnatural_Amino_Acids#Solid-phase_Synthesis_and_Chemical_Ligation UAA]])
** see [[PC]], [[PHP]], [[TE]], [[IPTG]], and [[Benzoyl]]
*** Also see [[Phosphoramidate]]

Latest revision as of 04:36, 23 August 2015

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