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| − | == Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
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| − | == Disclaimer ==
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| − | WOOP WOOP!
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| − | == Hypothesis ==
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| − | * GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]]
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| − | === D1210 perhaps just doesn't fit... ===
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| − | * I need a "non-leaky" strain which express the [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
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| − | ** lambda lysogen (ie HB101)
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| − | ** F+ plasmid
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| − | ** lacY
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| − | ** hsdR restriction system (ie: leaky restriction system)
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| − | ** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
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| − | === BW26434 ===
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| − | * Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
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| − | * However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
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| − | ** Are they doing this as a habit or training to become excessively insane?
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| − | ** A Cloning ninja signature perhaps ?
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| − | ** Random muslings ?
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| − | ** ?
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| − | ** WOOP WOOP !
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| − | * pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
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| − | * accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
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| − | * WOOP WOOP !!!
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| − | * PHP = amine orthogonal peptide protection group ? (see [[http://openwetware.org/wiki/CH391L/S12/Unnatural_Amino_Acids#Solid-phase_Synthesis_and_Chemical_Ligation UAA]])
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| − | ** see [[PC]], [[PHP]], [[TE]], [[IPTG]], and [[Benzoyl]]
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| − | *** Also see [[Phosphoramidate]]
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