Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation"

From OpenWetWare
Jump to: navigation, search
(Replacing page with '{{deleteme}}')
(14 intermediate revisions by the same user not shown)
Line 1: Line 1:
== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
== Disclaimer ==
== Hypothesis ==
* GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]]
=== D1210 perhaps just doesn't fit... ===
** I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
** lambda lysogen (ie HB101)
** F+ plasmid
** lacY
** hsdR restriction system (ie: leaky restriction system)
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
=== BW26434 ===
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
** Are they doing this as a habit or training to become excessively insane? 
** A Cloning ninja signature perhaps ?
** Random muslings ?
** ?

Latest revision as of 04:36, 23 August 2015

Crop.png It has been requested that this page be removed with restriction endonucleases.
Other articles for deletion are listed here.