Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation"

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== Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli ==
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{{deleteme}}
 
 
== Disclaimer ==
 
 
 
WOOP WOOP!
 
 
 
== Hypothesis ==
 
 
 
* GCG CAA ? [[http://cgsc.biology.yale.edu/Strain.php?ID=65553 1]]
 
 
 
=== D1210 perhaps just doesn't fit... ===
 
 
 
* I need a "non-leaky" strain which express the  [http://cgsc.biology.yale.edu/Mutation.php?ID=64657 araBAD] mutation.
 
** lambda lysogen (ie HB101)
 
** F+ plasmid
 
** lacY
 
** hsdR restriction system (ie: leaky restriction system)
 
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
 
 
 
=== BW26434 ===
 
 
 
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
 
* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
 
** Are they doing this as a habit or training to become excessively insane? 
 
** A Cloning ninja signature perhaps ?
 
** Random muslings ?
 
** ?
 
** WOOP WOOP !
 
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
 

Latest revision as of 04:36, 23 August 2015

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