Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo"

From OpenWetWare
Jump to: navigation, search
(Expected results)
(Replacing page with '{{deleteme}}')
 
(12 intermediate revisions by the same user not shown)
Line 1: Line 1:
== Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor ==
+
{{deleteme}}
 
 
* Date: 2012/12/03 10:29 AM EDT 
 
* Organism: E. coli (K-12 substr.)
 
* Strain: MG1655(?)
 
 
 
=== Hypothesis ===
 
 
 
Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
 
 
 
=== Experiment ===
 
 
 
==== Material ====
 
 
 
* 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
 
 
 
==== Expected results ====
 
 
 
* '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
 
* a heterocyclic base-labile group (NHC?)
 
* [[IPTG]] inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
 
** in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie [http://partsregistry.org/Part:BBa_V1003 laclq])
 
** From [[http://www.sigmaaldrich.com/life-science/molecular-biology/cloning-and-expression/learning-center/genotypes-of-e-coli.html Sigma]], the expected genotype matching the laclq strain is:
 
*** HB101 F– Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13h
 
 
 
==== References ====
 
 
 
* [https://www.openwetware.org/wiki/TE TE]
 
* [http://cgsc.biology.yale.edu/Strain.php?ID=9130 AB266]
 
* [http://cgsc.biology.yale.edu/Strain.php?ID=11958 E.coli B]: WOOP WOOP
 

Latest revision as of 03:39, 23 August 2015

Crop.png It has been requested that this page be removed with restriction endonucleases.
Other articles for deletion are listed here.