Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo"

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== Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor ==
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* Date: 2012/12/03 10:29 AM EDT 
 
* Organism: E. coli (K-12 substr.)
 
* Strain: MG1655(?)
 
 
 
=== Hypothesis ===
 
 
 
Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
 
 
 
=== Experiment ===
 
 
 
==== Material ====
 
 
 
* 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
 
 
 
==== Expected results ====
 
 
 
* '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
 
* a heterocyclic base-labile group (NHC?)
 
* [[IPTG]] inducible promoter: DNA lambda prophage induction (Transformation or mutation from lytic lacZ-repressed state to lysogenic!)
 
** in that case a matching E. coli genotype is "HB101 lacIq, lacY+" (ie [http://partsregistry.org/Part:BBa_V1003 laclq])
 
 
 
==== References ====
 
 
 
* [https://www.openwetware.org/wiki/TE TE]
 

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