Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:SolidPhasePeptideSynthesisReagentEvidencesInAgentScoobyDoo"

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== Measuring the amplitude of the synthetic reagent lysis TE buffer on NMDA receptor ==
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* Date: 2012/12/03 10:29 AM EDT 
 
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* Organism: E. coli
 
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* Strain:
 
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=== Hypothesis ===
 
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Amine protection groups (piperidine based) undergo chiral nucleophilic substitution on Histidine based autophosphorylation.
 
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=== Experiment ===
 
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==== Material ====
 
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* 1 x 10ml TE = TAE -> 10x buffer (Tris-HCl) (see [TE]
 
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==== Expected results ====
 
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* '''Amine''' protection groups = piperidine derived compound (NH2 undergo nucleophilic substitution on Histidine based autophosphorylation)
 
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* a heterocyclic base-labile group (NHC?)
 
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==== References ====
 
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* [https://www.openwetware.org/wiki/TE TE]
 

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