Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid cloning vectors for gene therapy with phosphohistidine based oligos"

From OpenWetWare
Jump to: navigation, search
(New page: = Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos = __TOC__ == Artificial DNA primers: phosphoramidate-based nucleosides synthesis == * N-6-benzoyl-deoxyadenos...)
 
(Artificial DNA primers: phosphoramidate-based nucleosides synthesis)
Line 1: Line 1:
 
= Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos =
 
= Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos =
 
__TOC__
 
__TOC__
== Artificial DNA primers: phosphoramidate-based nucleosides synthesis ==
+
== Artificial DNA primers: phosphoramidate-based oligonucleotide synthesis ==
  
 +
cDNA:
 
* N-6-benzoyl-deoxyadenosine phosphoramidite - Notice the P-N bound indicating the linkage of a phosphonate diester to a benzoyl atom :)
 
* N-6-benzoyl-deoxyadenosine phosphoramidite - Notice the P-N bound indicating the linkage of a phosphonate diester to a benzoyl atom :)
 
* N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent.
 
* N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent.
  
 
== References ==
 
== References ==

Revision as of 19:37, 5 November 2012

Plasmid cloning vectors for gene therapy with phosphohistidine-based oligos

Artificial DNA primers: phosphoramidate-based oligonucleotide synthesis

cDNA:

  • N-6-benzoyl-deoxyadenosine phosphoramidite - Notice the P-N bound indicating the linkage of a phosphonate diester to a benzoyl atom :)
  • N-3-oxo-hexanoyl-L-homoserine lactone - A light-inducible phosphohistidine-bound AHL ? A(denosine)TP/cAMP dependent.

References