User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation

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Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli




  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • hsdR restriction system (ie: leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP


  • Is described in details here:
  • However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
    • Are they doing this as a habit or training to become excessively insane?
    • A Cloning ninja signature perhaps ?
    • Random muslings ?
    •  ?
    • WOOP WOOP !
  • pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a cos mid)
  • accept inserts up to ~30 to 45 kb 1.
  • WOOP WOOP !!!
  • PHP = amine orthogonal peptide protection group ? (see UAA)