Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:Plasmid Strains carrying laclq mutation"

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(BW26434)
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** F+ plasmid  
 
** F+ plasmid  
 
** lacY
 
** lacY
** hsdR restriction system (ie: leaky restriction system)
+
** EGFR-hsdR restriction system (ie: a purely leaky restriction system)
 
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
 
** NO appropriate mutation of ARA operon/transport ! WOOP WOOP
  
=== BW26434 ===
+
=== Biobrick x BW26434 ===
  
 
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
 
* Is described in details here: http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks
* However I may miss a few details... Namely WTF is wrong with the peoples to forget thinking when its time for thinking.
 
** Are they doing this as a habit or training to become excessively insane? 
 
** A Cloning ninja signature perhaps ?
 
** Random muslings ?
 
** ?
 
** WOOP WOOP !
 
 
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
 
* pheS(mut) resistance gene inserts from pKSS plasmid... (I'm probably dealing with a [http://www2.wmin.ac.uk/~redwayk/lectures/vectors.htm cos mid])
* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1].
+
* accept inserts up to ~30 to 45 kb [http://xnet.rrc.mb.ca/davidb/cloning_vectors.htm 1]
* WOOP WOOP !!!
+
* Also see [[Phosphoramidate]].
* PHP = amine orthogonal peptide protection group ? (see [[http://openwetware.org/wiki/CH391L/S12/Unnatural_Amino_Acids#Solid-phase_Synthesis_and_Chemical_Ligation UAA]])
 
** see [[PC]], [[PHP]], [[TE]], [[IPTG]], and [[Benzoyl]]
 
*** Also see [[Phosphoramidate]]
 

Revision as of 13:51, 4 October 2013

Towards identification and full disclosure of mobile genetic elements inducible by Agent E.coli

Disclaimer

WOOP WOOP!

Hypothesis

  • GCG CAA ? [1]

D1210 perhaps just doesn't fit...

  • I need a "non-leaky" strain which express the araBAD mutation.
    • lambda lysogen (ie HB101)
    • F+ plasmid
    • lacY
    • EGFR-hsdR restriction system (ie: a purely leaky restriction system)
    • NO appropriate mutation of ARA operon/transport ! WOOP WOOP

Biobrick x BW26434