Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli:PhosphohistidineTransferaseComponent"
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| + | __TOC__ | ||
| + | == Agent Ecoli:Phosphohistidine Transferase Component == | ||
| − | + | === OmpR and LuxR === | |
| − | == | ||
| − | + | * Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this [http://2012.igem.org/Team:TU_Munich/Project/Caffeine page] for references. | |
| + | * In DNA/Purine deamination | ||
| + | ** http://www.kegg.jp/dbget-bin/www_bget?ko:K06223 (adenine-specific) | ||
| + | == See also == | ||
| − | * | + | * [http://openwetware.org/wiki/E._coli_restriction-modification_system E. coli restriction-modification system] |
| − | * | + | * [http://openwetware.org/wiki/Standard_E._coli_Strain_for_BioBricks Standard E. coli Strain for BioBricks] - and this [http://www.freepatentsonline.com/y2003/0138937.html paper] |
| + | * [http://openwetware.org/wiki/20.109(S07):Start-up_genome_engineering Start up genome engineering] [with the [http://www.ncbi.nlm.nih.gov/nuccore/U18997 M13] phage genome] - [a picture is [http://www.openwetware.org/images/1/1e/Macintosh_HD-Users-nkuldell-Desktop-GnmEng_coverart_S07.jpg HERE]] | ||
| + | * [http://openwetware.org/wiki/Beta-galactosidase_assay Beta-galactosidase assay] - EC 2.7.13.3 | ||
| + | * [http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/sequences/m13ko7.txt sequence for M13k07 reengineered plasmid] : '''Note''' : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting [http://www.uic.edu/classes/phar/phar331/lecture6/ plasmid cloning vector] will be a '''synthetic construct''' derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce '''horizontal''' gene regulation using bacterial conjugation like sex in elephants and the ''E. coli'' K-12 MG1655 strain. | ||
| + | * '''The Matrix (1999)''' | ||
Revision as of 07:55, 8 August 2013
Agent Ecoli:Phosphohistidine Transferase Component
OmpR and LuxR
- Orthogonal phosphorus-degrading enzymes are key in (auto)catalytic regulation of lacZ-bound phosphate-histidine residues - see N-methyl nucleosidase (3.2.2.35) and this page for references.
- In DNA/Purine deamination
- http://www.kegg.jp/dbget-bin/www_bget?ko:K06223 (adenine-specific)
See also
- E. coli restriction-modification system
- Standard E. coli Strain for BioBricks - and this paper
- Start up genome engineering [with the M13 phage genome] - [a picture is HERE]
- Beta-galactosidase assay - EC 2.7.13.3
- sequence for M13k07 reengineered plasmid : Note : If pMR101 is a gene vector derived from pACYC177 then clearly the resulting plasmid cloning vector will be a synthetic construct derived from a M13 like bacteriophage, thus the use of restriction enzymes for acquiring inter-species genes (OmpR/EnvZ) is likely a positive indicator of a scheme to induce horizontal gene regulation using bacterial conjugation like sex in elephants and the E. coli K-12 MG1655 strain.
- The Matrix (1999)
