Difference between revisions of "User:Etienne Robillard/Notebook/Agent Ecoli"

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= Agent Ecoli: The whys and wherefores of Angel Dust is in the product. =
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__TOC__
 
 
 
== Overview ==
 
 
 
__TOC__
 
 
 
* Long-term bioaerosols spraying (ie TL3')
 
* XXX: may carry a '''epigenetic trojan horse''' ?
 
* TL = toggle bistable switch / circuit
 
 
 
=== summary ===
 
 
 
Untested Agent E.coli 1.0 recipe:
 
 
 
* pick at least a combination of 1 (one) nerve agent (ie Benzoyl);
 
* autoclave custom plasmid(s) with ape... ;
 
* click on '''"I UNDERSTAND AND ACKNOWLEDGE THE RISKS OF CLONING FLUORESCENT ELEPHANTS, JUST GIMME SOME HARDCORE PLASMIDS TO MAKE MY CUSTOM OLIGOS!";
 
* add water (wash in bath with sodium hydrochloride solution..);
 
* To make a Lysis buffer reagent, add 1 (one) piperidine-hydrochloride derived reagent (ie Tris-HCl);
 
* To add a good source of aceton to your DNA mix, use 1 (one) 2-cyanoethyl derived reagent (acetonitrile);
 
* For doing oligos version, include 1 (one) organophosphate-based organic phosphatase compound (PHP, P-N, PC) as [http://www.organic-chemistry.org/protectivegroups/amino/boc-amino.htm base-labile] protection group...
 
* Moreover the DNA methylation process is believed to be regulated by endogenous enzymatic reactions implicated in phosphatase regulation.
 
 
 
Keywords: DNA methylation, cDNA, deoxyoligonucleotides, ribosomes, phosphatase (PAP2-like), Phosphohistidine regulation, Agent Dust, Agent Wet, PCP, Benzoyl, PHP, PC, lysogens, lytic cycle, trojan, horse, epygenetics, elephants, E. coli, K-12, MG1655, Agent Scooby Doo
 
 
 
== Introduction ==
 
 
 
[TODO]
 
 
 
== Chapters ==
 
 
 
=== Introduction to Agent E. coli ===
 
 
 
* Definition of Agent E.coli
 
** Basic description
 
** Geopolitical background
 
** Why is the term "chemtrails" inappropriate to describe domestic chemical warfare ?
 
*** It is my belief the "chemtrails" term is inappropriate to coherently define the existence of a long-term spraying operation of biologically active aerosols over my city. I believe the "chemtrails" terminology is in addition part of a propaganda scheme to deceit further independent scientists to examine the evidences towards the inclusion of mobile genetic elements being transfered into the human genome.
 
** Classification/Taxonomy
 
*** '''Agent E.coli 1.0''':
 
**** Class:  Nitrogenous-derived [http://en.wikipedia.org/wiki/Nerve_agent nerve precursor agent]. Exogenous compound (Military grade). Organophosphate based pesticide (phosphoramidate). Oil-in-water (O/W) nanoemulsion (Lysis Buffer reagents includes Triton X-100, Tween 80, Tris-HCl). Nitrogenous derived heterocyclic carbene (NHC).
 
 
 
=== Agent E. coli mecanics: Fundamental elements of domestic chemical warfare ===
 
 
 
* Synthetic biology and reverse/forward DNA transcription "circuits"
 
** luxR: Introduction to the Phosphohistidine transferase genetic switch
 
** [http://openwetware.org/index.php?title=User:Etienne_Robillard/Notebook/Agent_Ecoli:ToggleSwitchComponent Agent Ecoli:ToggleSwitchComponent] (TL phage lambda)
 
 
 
=== DNA evidences of synthetic nucleosides analogues are in the products ===
 
 
 
* dual input DNA promoters, heterocyclics, organocatalysts, and phosphoramidates-based nucleosides
 
* Plasmid addiction systems
 
** Tools (softwares): Ape (A Plasmid Editor), gibthon (a django-based web application)
 
** cDNA synthesis (RNAP)
 
==== Gibson assembly method ====
 
 
 
* Quote from the synbio.org.uk website: "Bill Collins contributed the development of a series of web-based tools for DNA assembly, including Gibthon - a software tool for automated design of the oligonucleotides required for Gibson Assembly." [[http://www.synbio.org.uk/dna-assembly/guidetogibsonassembly.html 1]]
 
** [2009-2010. see also the Team:Cambridge website for yet more connections to V. fischeri, a marine organism which expresses bioluminescence in dark environments.]
 
** Hopefully we sought not to encounter V. fischeri genes into elephants (or fast-running mouses!) except perhaps into Cambridge, USA: [[http://2010.igem.org/Team:Cambridge/Bioluminescence/G28 2]]
 
* See also [http://openwetware.org/wiki/Haynes:Gibson_Assembly Gibson Assembly], pSB3K3, BBa_J69512
 
* In summary, the Gibson assembly protocol provides an automated environment for template DNA synthesis (oligonucleotides) using a web-based interface written in Django for horizontal gene transfers into multi-plasmid cloning vectors such as the '''pSB3K3''' plasmid.
 
 
 
=== Forensic evidences of nitrogenous derived gene delivery on domestic civilian populations is a provocative and deliberate act of war on civilian liberties  ===
 
 
 
* Case-study of M13K07 derived genome engineering using site-directed mutagenesis
 

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