Difference between revisions of "User:Etienne Robillard/Notebook/30C6HSL/2013/03/18"

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m (Calmodulin kinase type II Beta subunit protein acquisition and expression with 30C6HSL based exogenous promoters (E. coli K-12))
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== Long-term effects of aerosol spraying on E. coli K-12 (MG1655) ==
=== Introduction to CAMK2B protein overexpression ===
* examine the acquisition and mutation of the CAMK2B gene in humans subject to horizontal 30C6HSL based transcription promoters (or "devices" for eugenic doctors).
* Calmodulin dependent inverse/split-pericams and CAMK2B interactions: CAMK2B may depend on exogenous promoters to convey the drug at the right time/place (site-directed mutagenesis). Notice that CAMK2B overexpression is probably an indicator of low neuronal activity (inverse pericams) or osmolitic regulator. (Critical sites: brain (frontal cortex region), amygdala and perhaps the hypothalamus)
* Never forget atmospheric NH2 as natural source of Nitrogen, hence a ethyl-methyl carbanion may be conjugated to a NH2 molecule in a process known as "benzoin condensation" to produce crystals (N-substituted benzoxazole heterocyclics), thus a form of artificial (asymmetric) [[User:Etienne_Robillard/Notebook/Schiff_base|Schiff base synthesis]] may occur as the result of injection of psychochemical intermediates into the atmosphere.
=== A synthetic biology approach to light-activated protein delivery : A review of recent advancements in synthetic mind control ===
* 2005 - The search for scFV osmolitic dimer -> ompC dimer protein:  [http://www.uniprot.org/uniprot/P06996.fasta P06996] [[http://openwetware.org/wiki/IGEM:MIT/2005 1]], [[http://www.ncbi.nlm.nih.gov/protein/56783237 2]], [[http://www.ncbi.nlm.nih.gov/nucleotide/49175990 3]]
* 2006 - The eau de coli experiment : ''Would E. coli, by any other name, smell as sweet?”''' : http://www.medgadget.com/2006/11/would_e_coli_by_1.html [http://openwetware.org/wiki/IGEM:MIT/2006 1]
** leucine auxotrophic gene insert -> osmY http://www.uniprot.org/uniprot/P0AFH8
** ''Complex transcriptional control of the sigma s-dependent stationary-phase-induced and osmotically regulated osmY (csi-5) gene suggests <font color="red">novel roles for Lrp, cyclic AMP (cAMP) receptor protein-cAMP complex, and integration host factor in the stationary-phase response of Escherichia coli.''</font> http://www.ncbi.nlm.nih.gov/pubmed/8253679
*** Leucine-responsive regulatory protein (in E.coli K12): See http://www.uniprot.org/uniprot/P0ACJ0
*** http://www.ncbi.nlm.nih.gov/nuccore/D11105 - Link to FASTA sequence file is [http://www.ncbi.nlm.nih.gov/nuccore/216585?report=fasta here].
* 2007 - ''Reversible acyl-homoserine lactone binding to purified Vibrio fischeri LuxR protein.'' http://www.ncbi.nlm.nih.gov/pubmed/14729687?dopt=Abstract and [[http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007 1]]
** ''Recovering patients often suffer <font color="red">hallucinations, and sometimes become psychotic</font>'' : http://www.openwetware.org/wiki/Image:IGEM2007_Imperial_iGEMGlobe.png
* 2008 -
* 2009 -
* 2010 - (current?), '''Unknown phenotype'''.  Homo sapiens (GRCh37.p10), s288c (Baker Yeast), E.coli BL21(DES3), E. coli, E. coli K12 (MG1655), W3110  : ''Berkeley Lab scientists open electrical link to living cells'' : http://www.eurekalert.org/pub_releases/2010-10/dbnl-bls102010.php
=== Calmodulin kinase type II Beta subunit protein acquisition and expression with 30C6HSL based exogenous promoters (E. coli K12) ===
* GO:1901896 positive regulation of calcium-transporting ATPase activity: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:1901896
* GO:0005513 detection of calcium ion: http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0005513
* GO:0006970 (osmY) : http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0006970
** Co-occuring terms/annotations for "osmY" are found [http://www.ebi.ac.uk/QuickGO/GTerm?id=GO:0006970#term=stats here].
* Significance of CAMK2B gene acquisition and amplification with exogenous 30C6HSL promoters : ''Within the rigid <font color="red">“β -can” structure (6) of GFP variants</font>, Baird et al. (7) found a site that would tolerate circular permutations where two portions of the polypeptide are flipped around the central site. With obvious clefts in the β-can, the chromophore of the circularly permuted GFPs (cpGFP) seemed to be <font color="red">more accessible to protons outside of the proteins</font>. The use of cpGFP might be interesting for converting the interaction signal between two protein domains into a change in the electrostatic potential of the chromophore; <font color="red">in other words, to transduce the information of the interaction into a fluorescent signal.</font>'' [[http://www.pnas.org/content/98/6/3197.full?sid=abc32731-4df0-45c4-bf46-7863678fb87c 1]]
* Browse the annotations for the Yeast (CMK1) ligand [http://www.uniprot.org/uniprot/P27466 here]. (EC
=== Motivations and purposes of psychochemical spraying in the morphogenesis of breast cancer, CES, Parkinson, and Alzheimer diseases via site-directed signaling (mutagenesis) ===

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