Difference between revisions of "User:Eric Ma/Notebook/iGEM 2010 Lab Prep/2010/05/13"

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==Entry title==
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==Restriction Enzyme Test==
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* To test restriction enzymes, digest DNA with E+P, X+S, E+S, X+P; in addition to this, do four single cuts.
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* We can tell which enzyme is not working (if it is such) by examining which pairs are not cutting properly.
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* I will cut Jason's miniprepped DNA because it had the most amount of DNA present.
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* 4 double digests + 4 single cuts, for further confirmation.
  
  

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Restriction Enzyme Test

  • To test restriction enzymes, digest DNA with E+P, X+S, E+S, X+P; in addition to this, do four single cuts.
  • We can tell which enzyme is not working (if it is such) by examining which pairs are not cutting properly.
  • I will cut Jason's miniprepped DNA because it had the most amount of DNA present.
  • 4 double digests + 4 single cuts, for further confirmation.