User:Eric Ma/Notebook/iGEM 2010 Lab Prep/2010/05/11: Difference between revisions
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* This is a good sign. Antibiotic batches are still working. | * This is a good sign. Antibiotic batches are still working. | ||
==Test of RFP-expression constructs== | |||
* Found old P<sub>con</sub>-RBS-RFP-Terminator construct from the Lagally Lab -80ºC freezer. And gave a diabolical laugh when I finally found it after digging through the two -80ºC freezers we used for a whole 30 minutes. | |||
* Inoculated in Amp100 +/- arabinose. | |||
* Control: No amp, no arabinose, expect growth; next time need a negative control. | |||
* Setup as follows: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Reagent''' | |||
| align="center" style="background:#f0f0f0;"|'''Tube A''' | |||
| align="center" style="background:#f0f0f0;"|'''Tube B''' | |||
| align="center" style="background:#f0f0f0;"|'''Tube C''' | |||
|- | |||
| Arabinose||+||-||- | |||
|- | |||
| Amp100||+||+||- | |||
|} | |||
* Results: | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Tube''' | |||
| align="center" style="background:#f0f0f0;"|'''Growth?''' | |||
| align="center" style="background:#f0f0f0;"|'''Red Coloration?''' | |||
|- | |||
| A||Yes||No | |||
|- | |||
| B||Yes||No | |||
|- | |||
| C||Yes||No | |||
|} | |||
* No red coloration observed when expected. Therefore, I will let the cultures grow a bit more to see if red coloration appears or not. | |||
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Revision as of 11:00, 12 May 2010
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Test of old BW27783 + PBAD-RBS-RFP-Term Cultures
Test of Old Antibiotic Stocks
Test of RFP-expression constructs
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