User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/04/23: Difference between revisions
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==Objective== | ==Objective== | ||
To conduct gel electrophoresis to test the activity of the DNase I attached to the enzyme. | |||
==Description== | ==Description== | ||
<u>Electrophoresis Gel Preparation</u. | |||
# Add 50 µL HPLC water to each of the lyophelized buffer/DNA samles. Try to get as much dissolved as possible. | |||
# Prepare a 1% agarose gel with 0.25g Agarose and 25mL TAE Buffer (microwaved together for 40 seconds). | |||
# Set in the electrophoresis tray with the comb for 20+ minutes. | |||
<u>Running The Gel</u> | |||
# A DNA control for the gel was prepared by placing 1 uL of DNA in 4 uL of water with 1 uL of loading dye | |||
# 5 uL of each sample was placed in a mini-pcr tube. 1 uL of loading dye was added to each tube | |||
# 6 uL of previously prepared ladder + dye were placed in the first lane. The other lanes were then filled with the samples prepared. | |||
# The lanes were loaded in the following order: 1) Ladder, 2) DNA alone 3) DNase I, 4) DNase I + 1 uL NHS Azide, 5) DNase I + 10 uL NHS Azide, 6) DNase I + 100 uL NHS Azide, 7) DNase I + 200 uL NHS Azide, 8) DNase I + 500 uL NHS Azide, 9) Hydrogel without DNase I, 10) DNA alone (repeated) | |||
# Once the gel was loaded, | |||
==Data== | ==Data== |
Revision as of 07:59, 3 May 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> |
ObjectiveTo conduct gel electrophoresis to test the activity of the DNase I attached to the enzyme. DescriptionElectrophoresis Gel Preparation</u.
Running The Gel
Data
NotesThis area is for any observations or conclusions that you would like to note.
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