Difference between revisions of "User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/04/22"

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
 
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Notes==
 
==Notes==
  
* This reaction was carried out at room temperature, not 37°C
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* This reaction was carried out at room temperature, not 37°C, which may have reduced or prevented enzymatic activity
  
  

Latest revision as of 21:40, 26 September 2017

BDLlogo notext lr.png Experimental Chemistry Report.pngMain project page
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Objective

To test the ability of the DNase I (attached to the hydrogels) to cleave DNA

Description

  1. In addition to the 5 samples of hydrogels with DNA, a sample of hydrogel without DNase and a sample of DNase I on its own were prepared as controls. The amount of DNase used for the control was equivalent to the amount of enzyme in the 500 uL hydrogel
  2. 1 uL of DNA from Chem 571 last year was added to each sample
  3. The DNA and DNase were mixed together for 3-4 hours.
  4. The hydrogels were then removed from the samples and the liquid was then frozen using liquid nitrogen. The sampes were then lyophilized overnight.

Data

No data was collected today.

Notes

  • This reaction was carried out at room temperature, not 37°C, which may have reduced or prevented enzymatic activity