User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/04/22: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
To test the ability of the DNase I (attached to the hydrogels) to cleave DNA | |||
==Description== | ==Description== | ||
# In addition to the 5 samples of hydrogels with DNA, a sample of hydrogel without DNase and a sample of DNase I on its own were prepared as controls. The amount of DNase used for the control was equivalent to the amount of enzyme in the 500 uL hydrogel | |||
# 1 uL of DNA from Chem 571 last year was added to each sample | |||
# The DNA and DNase were mixed together for 3-4 hours. | |||
# The hydrogels were then removed from the samples and the liquid was then frozen using liquid nitrogen. The sampes were then lyophilized overnight. | |||
==Data== | ==Data== | ||
No data was collected today. | |||
==Notes== | ==Notes== | ||
* This reaction was carried out at room temperature, not 37°C, which may have reduced or prevented enzymatic activity | |||
Latest revision as of 22:40, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
ObjectiveTo test the ability of the DNase I (attached to the hydrogels) to cleave DNA Description
DataNo data was collected today. Notes
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