User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/04: Difference between revisions
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<u>Starter Culture Preparation</u> | <u>Starter Culture Preparation</u> | ||
# | # Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes | ||
# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) | # To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) | ||
# Place the tubes on a shaker at 37°C overnight. | # Place the tubes on a shaker at 37°C overnight. | ||
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# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. | # Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. | ||
# The | # The solution was autoclaved for one hour | ||
# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | # Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | ||
#* This made about 20 agar plates | #* This made about 20 agar plates |
Revision as of 13:41, 8 March 2013
Experimental Chemistry | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveTo prepare agar plates and to prepare the starter culture of bacteria. DescriptionStarter Culture Preparation
Agar Plate Preparation
DataNo data was collected today Notes
To Do List this Week
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