User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/04: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<u>Starter Culture Preparation</u> | <u>Starter Culture Preparation</u> | ||
# | # Take out 4 aliquots of 4 mLs of broth from the 75 mL LB broth solution prepared last week and put into 4 test tubes | ||
# To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) | # To each tube, add bacteria by scraping frozen bacteria (BL21 E. coli) with a micropipette tip (should be about 5 uL). Do this in a sterile environment (flame) | ||
# Place the tubes on a shaker at 37°C overnight. | # Place the tubes on a shaker at 37°C overnight. | ||
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# Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. | # Agar plate solution was prepared by adding 12.5 g LB and 10 g agar to 500 mL water. | ||
# The | # The solution was autoclaved for one hour | ||
# Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | # Once the solution was cooled, 25 mL aliquots were poured into Petri dishes. | ||
#* This made about 20 agar plates | #* This made about 20 agar plates | ||
# The plates were left in the fridge to set | # The plates were left in the fridge to set | ||
==Data== | ==Data== |
Latest revision as of 22:30, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
ObjectiveTo prepare agar plates and to prepare the starter culture of bacteria. DescriptionStarter Culture Preparation
Agar Plate Preparation
DataNo data was collected today Notes
To Do List this Week
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