User:Elizabeth Ghias/Notebook/Experimental Chemistry/2013/03/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Experimental Chemistry</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Objective== | ==Objective== | ||
Prepare LB solutions for bacteria testing next week and crosslink the hydrogels that contain glutaraldehyde. | |||
==Description== | ==Description== | ||
<u>Analyze FTIR</u> | |||
* By looking at the FTIR spectra of our films, we determined that the quaternary ammonium salt was present in our film. Therefore we will move on to bacteria testing. | |||
<u>LB Broth Preparation</u> | |||
# Make one solution of 1.875 g LB broth in 75 mL water and make 8 solutions of 1.25 g LB broth in 50 mL water | |||
# Cap all with foil | |||
# Autoclave solutions for one hour | |||
<u>Hydrogel Autoclaving</u> | |||
# Allow the PVOH film containing glutaraldehyde to dry completely. | |||
# Prepare a solution of 100 mL H2O, 2 mL concentrated H2SO4, and 20 g Na2SO4 in a 150 mL beaker. (solution is referred to as glutaraldehyde fixant) | |||
# Place stir bar in beaker and a Buchner funnel top (cap) over the stir bar | |||
# Heat solution to 70°C (temperature on hotplate should be about 160) | |||
# Place film in solution for one hour. | |||
# Prepare 100 mL of a 1% HCl solution in a 150 mL beaker. Place a stir bar and an inverted buchner funnel into beaker. | |||
# Heat HCl solution to 70°C | |||
# Remove film from glutaraldehyde fixant and rinse with distilled water. | |||
# Place film into HCl solution for 30 minutes. | |||
# Prepare 0.2 M sodium bicarbonate solution. (1.68 g in 100 mL) | |||
# After 30 minutes, remove the film from the HCl solution and place into the sodium bicarbonate solution (at room temperature) for about 15 minutes. | |||
# Use tweezers to remove the film from the sodium bicarbonate solution. | |||
# Rinse film with deionized water. | |||
# The films were then allowed to dry over the weekend. | |||
==Data== | ==Data== | ||
No data was collected today. | |||
==Notes== | ==Notes== | ||
* When the hydrogels were crosslinked, they developed a fluid center, while having a squishy but somewhat dehydrated outside. After being left out to dry over weekend, the fluid center disappear and the films shrunk. | |||
Latest revision as of 22:32, 26 September 2017
Experimental Chemistry | Main project page Previous entry Next entry |
ObjectivePrepare LB solutions for bacteria testing next week and crosslink the hydrogels that contain glutaraldehyde. DescriptionAnalyze FTIR
DataNo data was collected today. Notes
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