User:Elizabeth Ghias/Notebook/Experimental Chemistry/2012/11/14: Difference between revisions
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==Objective== | ==Objective== | ||
Prepare 3 PVOH/glutaraldehyde films, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na<sub>2</sub>SO<sub>4</sub> | Prepare 3 PVOH/glutaraldehyde films, make another PVOH/Na-MT/glutaraldehyde film, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na<sub>2</sub>SO<sub>4</sub> | ||
==Description== | ==Description== | ||
<u>PVOH/glutaraldehyde | <u>PVOH/Glutaraldehyde Film Preparation</u> | ||
# 1 g PVOH was dissolved in 7 mL of water. | |||
# Once all the PVOH had dissolved, 1 mL of glutaraldehyde was added. | |||
# The solution was stirred for 5 minutes and then was poured into a dish and allowed to evaporate until Friday. | |||
<u>PVOH/Na-MT/Glutaraldehyde Film Preparation</u> | |||
# 1 g PVOH and 0.1 g Na-MT were dissolved in 7 mL H<sub>2</sub>O on a heated hot plate. | |||
# Once the PVOH dissolved, 1 mL of glutaraldehyde was added. | |||
# The gel was stirred for 3 minutes and then was poured into a plastic dish and was allowed to evaporate until Friday. | |||
<u>PVOH/Dye film preparation</u> | <u>PVOH/Dye film preparation</u> | ||
# | # Place 1 g PVOH in a small beaker | ||
#Dissolve in | # Dissolve in 7 mL of 0.1M NaHCO3/Na2CO3 buffer (pH=10) to deprotonate | ||
#Heat and | # Heat and stir to dissolve | ||
#Add | # Add 1 mg of dye (5-(4,6-Dichlorotiazinul Aminofluorescein) | ||
# | # Stir for 1 hr | ||
# Pour into dish | |||
# | |||
<u>0.01 M Na<sub>2</sub>SO<sub>4</sub> Solution Preparation</u> | |||
* 0.354 g Na<sub>2</sub>SO<sub>4</sub> was added to 500 mL water in a volumetric flask. | |||
==Data== | ==Data== | ||
* No data was collected today | |||
==Notes== | ==Notes== | ||
* The PVOH/dye solution turned a bright fluorescent yellow immediately as the dye was added. | |||
[[Category:Course]] | [[Category:Course]] |
Revision as of 09:13, 14 November 2012
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ObjectivePrepare 3 PVOH/glutaraldehyde films, make another PVOH/Na-MT/glutaraldehyde film, attach a dye to the PVOH in a film, and prepare a solution of 0.01 M Na2SO4 DescriptionPVOH/Glutaraldehyde Film Preparation
Data
Notes
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