User:Elizabeth Ghias/Notebook/Experimental Biological Chemistry/2011/11/16
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To synthesize nanoparticles and investigate the effects of changing the concentration of the reactants, while keeping the molar ratio constant, and to determine what solvents can be used to dissolve the purple fibers.
Four reactions were performed. Three epitube reactions and one large glass test tube reactions were set up. The large test tube reaction consisted of 1 mL of 30uM BSA, 0.5 mL of 4.17 mM HAuCl4, and 8.5 mL water. The tube was placed in an oven set to 70°C for 3 hours.
Epitube 1 - 100 uL BSA, 50 uL HAuCl4, and 850 uL water. Final Concentrations:
Epitube 2 - 25 uL HAuCl4, 50 uL BSA, and 925 uL water in this order. Final Concentrations:
Epitube 3 - 50 uL BSA, 25 uL HAuCl4, and 925 uL water in this order. Final Concentrations:
Solution 1 - 1 mL of 1 M Tris
Solution 2 - 1 mL of 6 M NaOH
Solution 3 - 1 mL of DMSO
Solution 4 - 1 mL of DMSO
A small clump of fibers was added to solutions 1, 2, and 3. Due to the success of dissolving fibers in solution 3, a large amount of fibers was added to solution 4.
This area is for any observations or conclusions that you would like to note.