Difference between revisions of "User:Eleni N. Kalivas/Notebook/CHEM-571/2013/10/30"

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(Objective)
(Protocol)
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==Protocol==
 
==Protocol==
Atomic Absorption
+
*5mL of each solution was prepared.
## centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
+
<br>
## make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
+
Preparation of Myoglobin-AuNP solutions
## Take measurements of your standards and samples
+
<br>
## Make a graph showing concentration of Au vs Au:protein ratio
+
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio Myoglobin'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount of gold added(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Myoglobin(M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Myoglobin(mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Water Myoglobin(mL)'''
 +
|-
 +
| 5||0.494264558||0.00005||2||2.505735442
 +
|-
 +
| 10||0.494264558||0.000025||1||3.505735442
 +
|-
 +
| 15||0.494264558||1.66667E-05||0.666666667||3.839068775
 +
|-
 +
| 20||0.494264558||0.0000125||0.5||4.005735442
 +
|-
 +
| 25||0.494264558||0.00001||0.4||4.105735442
 +
|-
 +
| 30||0.494264558||8.33333E-06||0.333333333||4.172402108
 +
|-
 +
| 35||0.494264558||7.14286E-06||0.285714286||4.220021156
 +
|-
 +
| 40||0.494264558||0.00000625||0.25||4.255735442
 +
|-
 +
| 45||0.494264558||5.55556E-06||0.222222222||4.28351322
 +
|-
 +
| 50||0.494264558||0.000005||0.2||4.305735442
 +
|}
 +
<br>
 +
Preparation of all Lysozyme-AuNP solutions
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Ratio Lysozyme'''
 +
| align="center" style="background:#f0f0f0;"|'''Amount gold added (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme(M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Lysozyme (mL)'''
 +
| align="center" style="background:#f0f0f0;"|'''Volume Water Lysozyme (mL)'''
 +
|-
 +
| 30||0.494264558||8.33333E-06||1.611148649||2.894586793
 +
|-
 +
| 50||0.494264558||0.000005||0.966689189||3.539046253
 +
|-
 +
| 70||0.494264558||3.57143E-06||0.690492278||3.815243164
 +
|-
 +
| 90||0.494264558||2.77778E-06||0.53704955||3.968685892
 +
|-
 +
| 110||0.494264558||2.27273E-06||0.439404177||4.066331265
 +
|-
 +
| 130||0.494264558||1.92308E-06||0.371803534||4.133931907
 +
|-
 +
| 150||0.494264558||1.66667E-06||0.32222973||4.183505712
 +
|-
 +
| 170||0.494264558||1.47059E-06||0.28432035||4.221415092
 +
|-
 +
| 190||0.494264558||1.31579E-06||0.254391892||4.25134355
 +
|-
 +
| 210||0.494264558||1.19048E-06||0.230164093||4.275571349
 +
|}
 +
<br>
 +
Stock Solution concentrations
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Concentration of Myoglobin (M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Gold(M)'''
 +
| align="center" style="background:#f0f0f0;"|'''Concentration Lysozyme (M)'''
 +
|-
 +
| 0.000125||0.00252901||2.58615E-05
 +
|}
 +
*All stock solutions were prepared with deionized H<sub>2</sub>O
 +
*The gold stock solution was prepared using Gold Chloride (II) trihydrate
 +
<br>
 +
Preparation of Lysozyme Stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Molecular Weight(g/mol)'''
 +
| align="center" style="background:#f0f0f0;"|'''14307'''
 +
|-
 +
| mass lysozyme(g)||0.0037
 +
|-
 +
| volume solution(mL)||10
 +
|-
 +
| Concentration Lysozyme (M)||2.58615E-05
 +
|}
 +
<br>
 +
Preparation of Myoglobin Stock solution
 +
<br>
 +
{| {{table}}
 +
| align="center" style="background:#f0f0f0;"|'''Molecular weight (g/mol)'''
 +
| align="center" style="background:#f0f0f0;"|'''17600'''
 +
|-
 +
| mass myoglobin (g)||0.022
 +
|-
 +
| volume solution (mL)||0.01
 +
|-
 +
| Concentration (M)||0.000125
 +
|}
 +
<br>
  
 
==Data==
 
==Data==

Revision as of 12:10, 30 October 2013

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Objective

The objective for today is to synthesize new Myoglobin-AuNP solutions with different ratios in order to determine the optimal concentration of Au:protein for the formation of fibers, and to also synthesize Lysozyme-AuNP solutions

Protocol

  • 5mL of each solution was prepared.


Preparation of Myoglobin-AuNP solutions

Ratio Myoglobin Amount of gold added(mL) Concentration Myoglobin(M) Volume Myoglobin(mL) Volume Water Myoglobin(mL)
5 0.494264558 0.00005 2 2.505735442
10 0.494264558 0.000025 1 3.505735442
15 0.494264558 1.66667E-05 0.666666667 3.839068775
20 0.494264558 0.0000125 0.5 4.005735442
25 0.494264558 0.00001 0.4 4.105735442
30 0.494264558 8.33333E-06 0.333333333 4.172402108
35 0.494264558 7.14286E-06 0.285714286 4.220021156
40 0.494264558 0.00000625 0.25 4.255735442
45 0.494264558 5.55556E-06 0.222222222 4.28351322
50 0.494264558 0.000005 0.2 4.305735442


Preparation of all Lysozyme-AuNP solutions

Ratio Lysozyme Amount gold added (mL) Concentration Lysozyme(M) Volume Lysozyme (mL) Volume Water Lysozyme (mL)
30 0.494264558 8.33333E-06 1.611148649 2.894586793
50 0.494264558 0.000005 0.966689189 3.539046253
70 0.494264558 3.57143E-06 0.690492278 3.815243164
90 0.494264558 2.77778E-06 0.53704955 3.968685892
110 0.494264558 2.27273E-06 0.439404177 4.066331265
130 0.494264558 1.92308E-06 0.371803534 4.133931907
150 0.494264558 1.66667E-06 0.32222973 4.183505712
170 0.494264558 1.47059E-06 0.28432035 4.221415092
190 0.494264558 1.31579E-06 0.254391892 4.25134355
210 0.494264558 1.19048E-06 0.230164093 4.275571349


Stock Solution concentrations

Concentration of Myoglobin (M) Concentration Gold(M) Concentration Lysozyme (M)
0.000125 0.00252901 2.58615E-05
  • All stock solutions were prepared with deionized H2O
  • The gold stock solution was prepared using Gold Chloride (II) trihydrate


Preparation of Lysozyme Stock solution

Molecular Weight(g/mol) 14307
mass lysozyme(g) 0.0037
volume solution(mL) 10
Concentration Lysozyme (M) 2.58615E-05


Preparation of Myoglobin Stock solution

Molecular weight (g/mol) 17600
mass myoglobin (g) 0.022
volume solution (mL) 0.01
Concentration (M) 0.000125


Data