User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/24
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Analyze the catalytic activity of pepsin in the presence and absence of pepstatin. The data collected today will be compared to our data taken from pepsin-AuNPs.
The following protocol was taken from Matt Hartings
We will allow the pepsin cleavage of hemoglobin to proceed for two hours with aliquots taken and analyzed every 1/2 hour. We will also run parallel reactions with different concentrations of pepstatin (pepsin inhibitor) present. We will save our aliquots for analysis tomorrow using SDS-PAGE.