Difference between revisions of "User:Eleni N. Kalivas/Notebook/CHEM-571/2013/09/17"

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(Objective)
(Objective)
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The objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide  K<sub>3</sub>[Fe(CN)<sub>6</sub>]. K<sub>3</sub>[Fe(CN)<sub>6</sub>], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite.  
 
The objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide  K<sub>3</sub>[Fe(CN)<sub>6</sub>]. K<sub>3</sub>[Fe(CN)<sub>6</sub>], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite.  
 
* the goal is to determine the endpoints of the oxidation and reduction of HRP.
 
* the goal is to determine the endpoints of the oxidation and reduction of HRP.
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==HRP Oxidation==
  
 
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Revision as of 10:37, 18 September 2013

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Objective

The objective of today's lab was to determine the amount of reagent necessary to fully reduce horseradish peroxidase (HRP). HRP oxidation will be done with potassium ferricyanide K3[Fe(CN)6]. K3[Fe(CN)6], which has a reduction potential of 424mV. HRP reduction will be done with Sodium Dithionite.

  • the goal is to determine the endpoints of the oxidation and reduction of HRP.

HRP Oxidation