# User:Elaine Marie Robbins/Notebook/CHEM-496/2011/12/07

< User:Elaine Marie Robbins‎ | Notebook‎ | CHEM-496‎ | 2011‎ | 12
Properties of AuNP V <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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## Objective

To try to control the aggregation process of AuNP/BSA.

## Description

1. Make 4 solutions of AuNP:
• Solutions 1 and 2 (Au/BSA mole ratio = 35)
1. Combine 0.5 mL of 2.5 mM HAuCl4 and 0.0706 mL of 0.0177 mM BSA in a test tube.
2. Add 5 mL of water.
3. Place in oven at 70°C.
• Solutions 3 and 4 (Au/BSA mole ratio = 100)
1. Combine 0.5 mL of 2.5 mM HAuCl4 and 2.02 mL of 0.0177 mM BSA in a test tube.
2. Add 5 mL of water.
3. Place in oven at 70°C.
2. Leave solutions 1 and 3 in oven for 3.5 hr.
3. Remove solutions 2 and 4 for 10 min every 30 min for 3.5 hr.

## Data

Solution 1: Au/BSA = 35, left in the oven

Solution 2: Au/BSA = 35, taken out of the oven every 30 minutes

Solution 3: Au/BSA = 100, left in the oven

Solution 4: Au/BSA = 100, taken out of the oven every 30 minutes

## Notes

Solution 1 began to turn purple after 2 hr.