User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/27
|AuNP III, Expression, DNA Transformation||<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page|
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To synthesize AuNP using the procedure developed here:
To begin culturing bacteria to express inteins.
To transform DNA coding for mutant GFP (mutated on 09/20/11) into Novablue cells.
The absorbance values for the AuNP solution were subtracted from the absorbance values for a blank acetate buffer to create a set of corrected absorbance values. The corrected absorbance values at 550 nm were graphed against time. The graph shows a positive linear relationship, indicating that AuNPs were formed in solution.
In addition, a second graph showing corrected absorbance against wavelength was created, to show the absorbance levels across all of the wavelengths measured by the UV-vis.
A magnified version of this graph shows that absorbance values at around 550 nm increased over time, indicating the creation of AuNPs.
Plate did not appear to be successful; no cells grew.
The AuNP solution turned from clear to purple while in the oven, indicating that AuNPs were actually formed.