User:Elaine Marie Robbins/Notebook/CHEM-496/2011/09/20
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To perform a PCR reaction to mutate green fluorescent protein (GFP) to contain a cysteine amino acid just after the enterokinase cleavage site.
Primer determined using http://www.basic.northwestern.edu/biotools/oligocalc.html:
Primer actually used in experiment:
Primers must have a melting point Tm ≥ 78°C, a GC content ≥ 40%, and be between 25-45 bases in length.
Sequence of GFP vector provided by Invitrogen.