Difference between revisions of "User:Easilva/Notebook/OEC isolations/2013/02/24"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> OEC_20130207F</span>
 
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
 
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==Entry title==
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==20130224==
* Insert content here...
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* Cells were feed and passaged - Both cells that were on 1:1 wells (21 Feb and 22 Feb) were passaged to T75 flasks - one normoxia conditions and the the other Hypoxia conditions. Also another, one well from cells, that were passaged before, but some remaining cells stayed behind did proliferated and were again passage for a T25 flask;
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* From normoxia, 5 out of 6 wells, did developed and isolated OECs (on day 17 since isolation);
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*From Hypoxia, 1 out of 6 wells, did developed and isolated OECs (on day 17 since isolation;
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* PW agreed to feed cells while I am out of town;
  
  

Revision as of 10:45, 24 February 2013

Owwnotebook icon.png OEC_20130207F <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>

20130224

  • Cells were feed and passaged - Both cells that were on 1:1 wells (21 Feb and 22 Feb) were passaged to T75 flasks - one normoxia conditions and the the other Hypoxia conditions. Also another, one well from cells, that were passaged before, but some remaining cells stayed behind did proliferated and were again passage for a T25 flask;
  • From normoxia, 5 out of 6 wells, did developed and isolated OECs (on day 17 since isolation);
  • From Hypoxia, 1 out of 6 wells, did developed and isolated OECs (on day 17 since isolation;
  • PW agreed to feed cells while I am out of town;