Difference between revisions of "User:Easilva/Notebook/HMVEC-D/2012/08/30"

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(Entry title)
 
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* 500,000 cells/vial - were generated 12 vials;
 
* 500,000 cells/vial - were generated 12 vials;
 
* 2.8X10<sup>6</sup> cells per flask, 86% viable;
 
* 2.8X10<sup>6</sup> cells per flask, 86% viable;
 +
* 16h26 cells went to -80˚C
  
 
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Latest revision as of 16:26, 30 August 2012

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Entry title

  • 15h20 cells were trypsinated and 1 flask was passage 1-4 xT75, the remaining 3 T75 flasks were freeze down for N2 storage (P5 cells)
  • 500,000 cells/vial - were generated 12 vials;
  • 2.8X106 cells per flask, 86% viable;
  • 16h26 cells went to -80˚C