# User:Dhea Patel/Notebook/Phosphorylation/2012/06/06

< User:Dhea Patel‎ | Notebook‎ | Phosphorylation‎ | 2012‎ | 06
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## Objective

Learn how to maintain an OpenWetWare Notebook.

## Description

1. Add experimental record here. Include what, how, and why...

## Data

Fractions 4-6

```     Ru complex+paper: 0.4826g
Weigh Paper: 0.2662g
Ru complex: 0.2164g
```

Fractions 7

```     Ru complex+paper: 0.3234g
Weigh Paper: 0.2612g
Ru complex: 0.0622g
```

Fractions and Observations

```  Fraction 4-6: Ru comp: 0.2164g | filtered clear liquid
Fraction 7: Ru comp: 0.0622g | filtered clear liquid
Fraction 8: Ru comp: 0.0028g | filtered yellow liquid
Fraction 1: Ru comp: 0.0213g | filtered yellow liquid
Fraction 2: Ru comp: 0.0219g |
Fraction 3: Ru comp: 0.0011g |
Fraction 9: Ru comp: 0.0004g |
Fraction 10: Ru comp: 0.0039g |
```

## Calculations

0.2164g ÷ (898.65g/mol) = 2.5E-4 mol --> 0.25 mmol Ru complex.

0.25 mmol Ru complex ÷ 5 equivalents = 0.05 mmol ATP

ATMP solution: 0.18 mmol × 5 equivalents = 0.9 mmol --> 1 mmol

5E-5 mol ATP × 507.18 g/mol = 0.02445 g ATP

Predicting product mass

RuComplex: RuC32H25N7 P2F12

ATP: C10H12N5 O12P3

RuATP: RuC42H37N12O12P5F12

```Ru - 101.17 g/mol
C - 504.42 g/mol
H - 37.37  g/mol
N - 168.12 g/mol
O - 192    g/mol
P - 154.85 g/mol
F - 228    g/mol
Total - 1385.83 g/mol
```

## Notes

This area is for any observations or conclusions that you would like to note.