Difference between revisions of "User:Dhea Patel/Notebook/Hemoglobin Project/2013/06/13"

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m (Data)
m (Data)
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The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned.  
 
The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned.  
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<b>Mb/Phosphate in Hexane</b>
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[[Image:Mb_Phosphate_OPD_H2O2_Hexane_WORKUP_GRAPH.bmp]]
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<b>Mb/Acetate in Hexane</b>
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[[Image:Mb_Acetate_OPD_H2O2_Hexane_WORKUP_GRAPH.bmp]]
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Note: The high absorbance peak at 140 minutes is due to a potential water/protein precipitate that filtered through.
  
 
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Revision as of 12:17, 13 June 2013

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Reaction of OPD+H2O2 with Mb Catalyst in Acetone Suspension

The procedurefor the reaction of OPD and H2O2 was followed using a Mb/Tris catalyst and an acetone suspension. The reaction was run in a beaker with a stir bar and the solution was centrifuged for 1 minute at 13,200 rpm before taking a spectrum.

In addition reactions were run using Mb/Phosphate and Mb/Acetate in hexanes suspension. These samples were filtered (not centrifuged) before taking a spectrum.

Data

Mb/Tris in Acetone, Trial 1 Mb Tris1 OPD H2O2 Acetone WORKUP GRAPH.JPG

The drop in absorbance at 140 minutes is due to the outside of the cuvette being cleaned.

Mb/Tris in Acetone, Trial 2 Mb Tris2 OPD H2O2 Acetone WORKUP GRAPH.JPG

The drop in absorbance at 120 minutes and 140 minutes is due to the outside of the cuvette being cleaned.

Mb/Phosphate in Hexane Mb Phosphate OPD H2O2 Hexane WORKUP GRAPH.bmp

Mb/Acetate in Hexane Mb Acetate OPD H2O2 Hexane WORKUP GRAPH.bmp

Note: The high absorbance peak at 140 minutes is due to a potential water/protein precipitate that filtered through.