Difference between revisions of "User:Dhea Patel/Notebook/Hemoglobin Project/2013/04/02"

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==Protocol==
 
==Protocol==
 
*15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
 
*15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
*8.8mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
+
*8.9mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
 
*1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.
 
*1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.
  

Revision as of 06:54, 2 April 2013

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Protocol

  • 15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
  • 8.9mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
  • 1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.
  • UV-vis was run on the Mb in MeOH solution after it had been centrifuged to determine how much Mb was in solution.