User:Dhea Patel/Notebook/Hemoglobin Project/2013/04/02: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
mNo edit summary
mNo edit summary
Line 8: Line 8:
==Protocol==
==Protocol==
*15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
*15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
*8.8mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
*8.9mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
*1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.
*1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.



Revision as of 06:54, 2 April 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Protocol

  • 15.2mL MeOH was added to 16.4mg o-Phenylenediamine.(10mM)
  • 8.9mg Myoglobin, KCl in Citrate (pH3) was added to 1mL MeOH.
  • 1.2mL Hydrogen Peroxide was added to MeOH to make a 1M stock solution.
  • UV-vis was run on the Mb in MeOH solution after it had been centrifuged to determine how much Mb was in solution.