Difference between revisions of "User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/26"

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(Autocreate 2013/02/26 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project)
 
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==Entry title==
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==Objective==
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*to run UV-vis on 11.6mg p-sorbitol in Citrate Buffer in MeOH at temperatures ranging from 5°C to 50°C, with 5°C increments.
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==Protocol==
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* MeOH blank was run in UV-vis and Fluorescence.
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* 11.6mg of myoglobin with KCl in 20mL 0.01M pH 3 citrate buffer (evaporated) made [[0.0058g Myoglobin + 0.4961g KCl in 20mL 0.01M pH 3 citrate buffer (evaporated)| January 30th]] was run in UV-vis and Fluorescence.
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*The data was analyzed and compared to the data from January 30th. To find that the UV-vis absorbance was the same, but the Fluorescence had a greater intensity.
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[[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png]] [[Image:UV-vis_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]]
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[[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH%2C_taken_February_26%2C_2013.png]] [[Image:Fluorescence_of_11.6mg_Citrate_Solid_in_1mL_MeOH.png]]
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Revision as of 07:23, 26 February 2013

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Objective

  • to run UV-vis on 11.6mg p-sorbitol in Citrate Buffer in MeOH at temperatures ranging from 5°C to 50°C, with 5°C increments.

Protocol

  • MeOH blank was run in UV-vis and Fluorescence.
  • 11.6mg of myoglobin with KCl in 20mL 0.01M pH 3 citrate buffer (evaporated) made January 30th was run in UV-vis and Fluorescence.
  • The data was analyzed and compared to the data from January 30th. To find that the UV-vis absorbance was the same, but the Fluorescence had a greater intensity.


UV-vis of 11.6mg Citrate Solid in 1mL MeOH, taken February 26, 2013.png UV-vis of 11.6mg Citrate Solid in 1mL MeOH.png

Fluorescence of 11.6mg Citrate Solid in 1mL MeOH, taken February 26, 2013.png Fluorescence of 11.6mg Citrate Solid in 1mL MeOH.png