User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15

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Objective

  • Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.

Description

  • 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
  • 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
  • 10.5mg mannitol in tris buffer, solvent: ethyl acetate
  • 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
  • 11.7mg mannitol in tris buffer, solvent: water
  • 10.0mg p-sorbitol in phosphate buffer, solvent: water
  • 11.6mg mannitol in tris buffer, solvent: chloroform
    • centrifuged for an additional 10 minutes
  • 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform


  • all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.