Difference between revisions of "User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15"

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(Autocreate 2013/02/15 Entry for User:Dhea_Patel/Notebook/Hemoglobin_Project)
 
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==Entry title==
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==Objective==
* Insert content here...
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* Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.
  
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==Description==
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*10.5mg mannitol in Tris buffer, solvent: Acetonitrile
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*10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
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*10.5mg mannitol in tris buffer, solvent: ethyl acetate
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*12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
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*11.7mg mannitol in tris buffer, solvent: water
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*10.0mg p-sorbitol in phosphate buffer, solvent: water
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*11.6mg mannitol in  tris buffer, solvent: chloroform
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**centrifuged for an additional 10 minutes
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*10.0mg p-sorbitol in phosphate buffer, solvent: chloroform
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*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
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*Centrifuge settings were as follows:
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**13200rpm
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**0:16 seconds
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**851 rotor
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*all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
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*Each solvent was used as a blank.
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*The data was corrected for the blank and a corrected baseline.
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*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.
  
 
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Revision as of 06:58, 15 February 2013

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Objective

  • Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.

Description

  • 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
  • 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
  • 10.5mg mannitol in tris buffer, solvent: ethyl acetate
  • 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
  • 11.7mg mannitol in tris buffer, solvent: water
  • 10.0mg p-sorbitol in phosphate buffer, solvent: water
  • 11.6mg mannitol in tris buffer, solvent: chloroform
    • centrifuged for an additional 10 minutes
  • 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform


  • all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.