User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15

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Objective Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform. Description 10.5mg mannitol in Tris buffer, solvent: Acetonitrile 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile 10.5mg mannitol in tris buffer, solvent: ethyl acetate 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate 11.7mg mannitol in tris buffer, solvent: water 10.0mg p-sorbitol in phosphate buffer, solvent: water 11.6mg mannitol in tris buffer, solvent: chloroform centrifuged for an additional 10 minutes 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform all the mannitol in tris buffer samples were vortexed and centrifuged before being measured Centrifuge settings were as follows: 13200rpm 0:16 seconds 851 rotor all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents. Each solvent was used as a blank. The data was corrected for the blank and a corrected baseline. Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.