Difference between revisions of "User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15"

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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Latest revision as of 21:27, 26 September 2017

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  • Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform.


  • 10.5mg mannitol in Tris buffer, solvent: Acetonitrile
  • 10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile
  • 10.5mg mannitol in tris buffer, solvent: ethyl acetate
  • 12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate
  • 11.7mg mannitol in tris buffer, solvent: water
  • 10.0mg p-sorbitol in phosphate buffer, solvent: water
  • 11.6mg mannitol in tris buffer, solvent: chloroform
    • centrifuged for an additional 10 minutes
  • 10.0mg p-sorbitol in phosphate buffer, solvent: chloroform

  • all the mannitol in tris buffer samples were vortexed and centrifuged before being measured
  • Centrifuge settings were as follows:
    • 13200rpm
    • 0:16 seconds
    • 851 rotor
  • all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents.
  • Each solvent was used as a blank.
  • The data was corrected for the blank and a corrected baseline.
  • Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min.