User:Dhea Patel/Notebook/Hemoglobin Project/2013/02/15: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==Objective== | ||
* | * Run Fluorescence on p-sorbitol in phosphate buffer and mannitol in tris buffer samples in either acetonitrile, ethyl acetate, water, or chloroform. | ||
==Description== | |||
*10.5mg mannitol in Tris buffer, solvent: Acetonitrile | |||
*10.8mg p-sorbitol in phosphate buffer, solvent: Acetonitrile | |||
*10.5mg mannitol in tris buffer, solvent: ethyl acetate | |||
*12.1mg p-sorbitol in phosphate buffer, solvent: ethyl acetate | |||
*11.7mg mannitol in tris buffer, solvent: water | |||
*10.0mg p-sorbitol in phosphate buffer, solvent: water | |||
*11.6mg mannitol in tris buffer, solvent: chloroform | |||
**centrifuged for an additional 10 minutes | |||
*10.0mg p-sorbitol in phosphate buffer, solvent: chloroform | |||
*all the mannitol in tris buffer samples were vortexed and centrifuged before being measured | |||
*Centrifuge settings were as follows: | |||
**13200rpm | |||
**0:16 seconds | |||
**851 rotor | |||
*all the p-sorbitol in phosphate buffer samples were sonicated for 3 hours in their respective solvents. | |||
*Each solvent was used as a blank. | |||
*The data was corrected for the blank and a corrected baseline. | |||
*Fluorescence was run with an emission scan of 310-550nm at an excitation of 290nm and scan speed of 100nm/min. | |||
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Latest revision as of 22:27, 26 September 2017
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